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派洛宁Y
产品纠错
CAS号:92-32-0 | 英文名称:Pyronin Y
分子式 C17H19ClN2O
分子量 303
EINECS号 202-147-8
MDL MFCD00011725
Smiles C12C=C(C=CC=1C=C1C=C/C(=[N+](\C)/C)/C=C1O2)N(C)C.[Cl-]
InChIKey INCIMLINXXICKS-UHFFFAOYSA-M
乙二醇化学百科
基本信息
中文名称 吡啰红Y
英文名称 PYRONIN Y
CAS号 92-32-0
分子式 C17H19ClN2O
分子量 302.8
EINECS号 202-147-8
物化性质
熔点 250-260 °C(lit.)
溶解度 易溶于水;微溶于乙醇、乙二醇、甲基溶纤剂
色指数 45005
形态 粉末/固体
颜色 粉色
水溶解性 Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
最大波长(λmax) 548 nm
Merck 13,8097
BRN 3795789
稳定性 稳定的。与强氧化剂不相容。
生物领域应用 Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides
安全信息
危险品标志 Xn,Xi
危险类别码 68-36/37/38
安全说明 22-24/25-36-26
WGK Germany 3
RTECS号 BQ1450000
TSCA Yes
海关编码 32041300
生产及用途
Pyronin Y (Pyronine G) 是一种可插入 RNA 的阳离子染料,可以靶向包括 RNA,DNA 和细胞器的细胞结构。Pyronin Y 与双链核酸(尤其是 RNA) 形成荧光复合物,可以对细胞 RNA 进行半定量分析。Pyronin Y 可用于鉴定活细胞中核糖核蛋白复合物的特定 RNA 亚种。

Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).

用途 
一种插入RNA的阳离子染料,在电泳过程中呈红色带。
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产品供应商
92-32-0
上海阿拉丁生化科技股份有限公司
2023-10-02

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