CasImg
尼罗丝
产品纠错
CAS号:7385-67-3 | 英文名称:Nile Red
分子式 C20H18N2O2
分子量 318
EINECS号 230-966-0
MDL MFCD00011639
Smiles
InChIKey
乙二醇化学百科
基本信息
中文名称 尼罗红
英文名称 Nile Red
CAS号 7385-67-3
分子式 C20H18N2O2
分子量 318.37
EINECS号 230-966-0
InChI InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
SMILES C12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1
物化性质
熔点 203-205 °C(lit.)
沸点 457.53°C (rough estimate)
密度 1.1871 (rough estimate)
折射率 1.5700 (estimate)
溶解度 在甲醇中可溶1mg/mL
形态 粉末
酸度系数(pKa) 4.08±0.20(Predicted)
颜色 深红色至栗色
BRN 279110
稳定性 稳定的。与强氧化剂不相容。
安全信息
安全说明 24/25
WGK Germany 3
F 8-10
海关编码 29349990
生产及用途
Nile Red (Nile Blue A oxazone, Phenoxazone 9)是一种十分有效的荧光染料,可在疏水环境下检测细胞内脂质滴 intracellular lipid droplets。Nile Red 可用于细胞内脂质,蛋白质的疏水域和溶酶体磷脂内含体的染色。
TargetValue
lipid droplet
()

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.

用途 
生化研究
产品供应商
7385-67-3
上海阿拉丁生化科技股份有限公司
2023-10-02

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