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CAS号:7385-67-3
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英文名称:Nile Red
分子式
C20H18N2O2
分子量
318
EINECS号
230-966-0
MDL
MFCD00011639
Smiles
InChIKey
乙二醇化学百科
基本信息
物化性质
安全信息
生产及用途
Nile Red (Nile Blue A oxazone, Phenoxazone 9)是一种十分有效的荧光染料,可在疏水环境下检测细胞内脂质滴 intracellular lipid droplets。Nile Red 可用于细胞内脂质,蛋白质的疏水域和溶酶体磷脂内含体的染色。
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.
用途 生化研究
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