| 中文名称 | 1,4-二亞硝哌【口+井】 |
| 英文名称 | N,N'-DINITROSOPIPERAZINE |
| CAS号 | 140-79-4 |
| 分子式 | C4H8N4O2 |
| 分子量 | 144.13 |
| EINECS号 | 205-434-6 |
| 熔点 | 208-210°C |
| 沸点 | 262.72°C (rough estimate) |
| 密度 | 1.4165 (rough estimate) |
| 折射率 | 1.8500 (estimate) |
| 溶解度 | 可溶于氯仿（少许）、DMSO（少许）、甲醇（少许） |
| 形态 | 固体 |
| 酸度系数(pKa) | -4.82±0.70(Predicted) |
| 颜色 | 白色至浅黄色 |
| 水溶解性 | 5.765g/L(24 ºC) |
| 稳定性 | 吸湿性 |
| 毒害物质数据 | 140-79-4(Hazardous Substances Data) |
N,N'-Dinitrosopiperazine (0.5-100 μM; 48 hours) has no inhibitory effects on the labeled 6-10B cells, and LDH activity is not significantly altered by DNP treatment in the 0.5-8 μM concentration range. However, it is cytotoxic from the concentration 10 μM.N,N'-Dinitrosopiperazine (2-8 μM; 24 hours) induces 6-10B cell invasion and motility in a dose-dependent manner. At 6 μM, when compares to the control group, DNP increases cell invasion at 421.7% and cell motility is increased by 328.2%.N,N'-Dinitrosopiperazine (6 μM; 24 hours) increases the expression of phospho-LYRIC s568 and LYRIC expression in CNE1 cells.
Cell Viability Assay
| Cell Line: | The labeled 6-10B cells |
| Concentration: | 0.5, 1, 2, 4, 6, 8, 10, 20, 40, 80, or 100 μM |
| Incubation Time: | 48 hours |
| Result: | Had no inhibitory effects at the concentration 0-8 μM. |
Western Blot Analysis
| Cell Line: | The NPC cell line CNE1 |
| Concentration: | 6 μM |
| Incubation Time: | 24 hours |
| Result: | Increased phospho-LYRIC s568 and LYRIC expression. |
N,N'-Dinitrosopiperazine (injected into the tail veins; 40 mg/kg; 30 days) inhibits cell motility and invasion, and facilitates NPC metastasis in vivo. From a IHC result, Phospho-LYRIC expression is higher in the metastatic tumors of DNP-treated mice than in those of the untreated control mice.
| Animal Model: | BABL/c nude mice injected with labeled 6-10B cell suspensions (1 × 10 4 cells) with or without DNP(40 mg/kg) |
| Dosage: | 40 mg/kg |
| Administration: | Injected into the tail veins; 30 days |
| Result: | Induced LYRIC phosphorylation at serine 568 associated with NPC metastasis in vivo. |