| 中文名称 | 5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮 |
| 英文名称 | SMI-4a |
| CAS号 | 327033-36-3 |
| 分子式 | C11H6F3NO2S |
| 分子量 | 273.23 |
| EINECS号 | 200-258-5 |
| 熔点 | 181-183℃ (methanol ) |
| 密度 | 1.530±0.06 g/cm3(Predicted) |
| 溶解度 | ≥ 9.4mg/mL，溶于 DMSO |
| 形态 | 白色至类白色固体。 |
| 酸度系数(pKa) | 7.26±0.20(Predicted) |
| 危险类别码 | 36-43 |
| 安全说明 | 26-36/37 |
Pim
TCS-PIM-1-4a (10 μM; 24-48 hours; 6812/2 cells and Jurkat cells) treatment increases the population of cells in the G1 phase from 44.3% to 68.4% and from 56.2% to 67.1% in 6812/2 and Jurkat, respectively. S-phase cells are decreased in 6812/2, whereas only small changes are seen in Jurkat cells consistent with the lesser G1 block.
TCS-PIM-1-4a (5 μM; 6 hours; 6812/2 cells and Jurkat cells) induces cell death by the induction of apoptosis.
TCS-PIM-1-4a (5 μM; 4-8 hours; 6812/2 cells and Jurkat cells) prevents the increase in 4E-BP1 protein levels and inhibits mTOR-directed phosphorylation on Thr37/46.
Cell Cycle Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Induced cell-cycle arrest. |
Apoptosis Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 5 μM |
| Incubation Time: | 6 hours |
| Result: | Led to an increase in the number of the cells positive for annexin V and negative for PI from 8.25% in the control to 21.85%. |
Western Blot Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 10 μM |
| Incubation Time: | 4 hours, 8 hours |
| Result: | Prevented the increase in 4E-BP1 protein levels and inhibited mTOR-directed phosphorylation on Thr37/46. |
TCS-PIM-1-4a (SMI-4a; 60 mg/kg; oral gavage; twice daily; for 21 days; nu/nu nude mice) treatment causes a significant delay in the tumor growth without any change in the weight, blood counts, or chemistries.
| Animal Model: | 18 Nu/nu nude mice with 6812/2 murine pre–T-LBL cells |
| Dosage: | 60 mg/kg |
| Administration: | Oral gavage; twice daily; for 21 days |
| Result: | Caused a significant delay in the tumor growth. |
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CAS号:226907-52-4
6-氨基-7,9-二氢-2-(2-甲氧基乙氧基)-9-(苯甲基)-8H-嘌呤-8-酮 -
CAS号:432020-20-7
(4-Chloro-3-nitrophenyl)(4-(2-fluorophenyl)piperazin-1-yl)Methanone -
CAS号:88-05-1
2,4,6-三甲基苯胺 -
CAS号:380623-76-7
SM 7368 -
CAS号:587852-28-6
SMI-16a -
CAS号:2489-03-4
三溴甲基苯 -
CAS号:473930-22-2
SM-276001 -
CAS号:226907-52-4
6-氨基-7,9-二氢-2-(2-甲氧基乙氧基)-9-(苯甲基)-8H-嘌呤-8-酮 -
CAS号:432020-20-7
(4-Chloro-3-nitrophenyl)(4-(2-fluorophenyl)piperazin-1-yl)Methanone -
CAS号:88-05-1
2,4,6-三甲基苯胺 -
CAS号:380623-76-7
SM 7368 -
CAS号:587852-28-6
SMI-16a -
CAS号:2489-03-4
三溴甲基苯 -
CAS号:473930-22-2
SM-276001